OCT test targets and phantoms

Calibrate, validate, and compare OCT systems with tissue-mimicking test targets and phantoms. Choose from multilayer, PSF, pyramid, and USAF targets to test resolution, distortion, focus, alignment, and reference stability.

BioPixS OCT test targets and phantoms are designed to help you quantify OCT performance in a repeatable way. In other words, they turn “it looks good” into numbers you can track over time, compare across instruments, or report in technical documentation.

Different OCT validation tasks require different geometries, hence this category includes four complementary standards: a multilayer target (depth validation), a PSF target (spot response), a pyramid target (3D step geometry), and a USAF target (lateral resolution and alignment). Whether you are trying to test lateral resolution or PSF, we’ve got you covered!

What these OCT targets help you test

• Lateral resolution (USAF groups + line pairs) and practical “what can I resolve?” checks.

• Axial / depth performance (layered structures and step geometry).

• PSF / spot response for benchmarking optics + processing, and for tracking drift.

• Distortion & scaling (straight features and repeatable geometry), so you can validate corrections.

• Focus, field-of-view, and uniformity, especially when you need confidence across the full scan area.

• Alignment & referencing, so that routine QA becomes faster and more repeatable.

Product range at a glance

OCT Multilayer Test Targets – For depth resolution and contrast-versus-depth validation using repeatable multilayer structures.

OCT PSF Test Target – For axial/lateral resolution and focus checks using FeO nanoparticle scatterers inside a polymer cylinder.

OCT Pyramid Test Targets – For resolution, distortion, reference, focus and field-of-view testing using stepped pyramid structures (positive/negative options).

OCT USAF Test Targets – For lateral resolution, distortion, alignment and calibration using USAF Groups 2–8, PSF features, Ronchi rulings, and alignment markers (positive/negative; glossy/matte).

Product	Best for	What you can measure	What makes it different
OCT Multilayer Test Targets	Depth and layer visibility validation	Layer separation, depth scaling, depth uniformity	Multi-layer structure for depth-oriented checks
OCT PSF Test Target	PSF benchmarking and stability tracking	PSF width/shape, system blur, repeatability over time	Compact cylinder; designed to isolate PSF behaviour
OCT Pyramid Test Targets	3D geometry validation	Focus, FOV, distortion, spatial/axial resolution behaviour on steps	Micron-scale step pyramid geometry (positive/negative options)
OCT USAF Test Target (Group 2–8)	Lateral resolution + alignment + calibration	Resolution groups, Ronchi rulings, alignment, PSF features	Chrome USAF pattern + alignment markers + Ronchi (10/100 lines/mm)

Quick selection guide

If your main goal is…	Choose…	Why
Depth resolution and contrast vs depth	OCT Multilayer	Known layer count/thickness enables depth benchmarking.
Extract PSF / verify focus	OCT PSF	FeO nanoparticles provide point-like scatterers for PSF analysis.
Check distortion, FoV, focus across the scan	OCT Pyramid	3D stepped geometry highlights non-linearity and field effects.
Validate lateral resolution + alignment + calibration	OCT USAF	USAF + Ronchi + markers make resolution and alignment checks straightforward.

Typical workflows

• Incoming QA / routine QC: scan the same region and trend key metrics over time (resolution, distortion, contrast vs depth).

• System comparison: image two OCT systems with identical settings and then compare outputs using the same target.

• Algorithm validation: use repeatable geometry to validate segmentation, deconvolution, distortion correction, and also focus optimization.

Why choose BioPixS OCT test targets

• Designed for OCT validation, not generic imaging: therefore, we chose geometry and materials for practical OCT workflows.

• Tissue-mimicking approach: many targets use phantom substrates so the measurement conditions are closer to real samples.

• Traceability mindset: spec sheets + batch/trace codes help you document and reproduce results (useful for QA and publications).

• A coherent set: the four products are meant to work together, so you can expand from “one check” to “full performance mapping” without changing supplier.

Download the comparison guide here!