Fluorescence Lifetime Imaging (FLIM) is increasingly used in biomedical imaging and clinical diagnostics, but many systems don’t require ultra-fine resolution for effective calibration, that’s where our Low Resolution FLIM Test Targets come in — a cost-effective, lifetime-calibrated solution built for standard-resolution imaging platforms.
Designed using USAF resolution patterns ranging from Group -2 to 5 or 0 to 5, and embedded with characterized fluorescent dyes (BPXS1LT, BPXS3LT, BPXS4LT, BPXS5LT), these targets offer traceable fluorescence lifetimes in a format optimized for lower-resolution FLIM systems. They’re ideal for system validation, software testing, training environments, and labs with budget or resolution constraints.
Download the pdf with the emission and excitation spectra here
Key features & benefits
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Resolution Groups -2 to 5 or 0 to 5: Designed for systems that don’t require high-end resolution limits.
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Lifetime-Characterized Dyes: Includes BPXS1LT, BPXS3LT, BPXS4LT, and BPXS5LT, each with stable and documented lifetimes.
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Validated for FLIM: Compatible with confocal, wide-field, and point-scan systems using TCSPC or time-gated detection.
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Budget-Friendly Format: Offers the essential benefits of FLIM calibration at a more accessible price point.
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Positive or Negative Contrast Available: Supports varied imaging configurations and detection geometries.
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Long-Term Performance: Lifetimes remain stable over extended periods, validated for over one year.
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Easy to Integrate: No system modification required—ready to use with standard stages and holders.
Why Choose Low Resolution Fluorescence Lifetime test targets
Not every FLIM system needs to push the limits of high spatial resolution—but all of them need reliable, reproducible calibration. These low resolution fluorescence lifetime test targets fill a critical gap for users who value:
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Affordability without compromising quality
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Valid lifetime benchmarks for quantitative imaging
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A tool that supports routine quality control across different platforms
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A reproducible solution backed by long-term stability studies
Until now, labs often relied on unstable liquid dye samples or custom setups. This is the first commercial solution offering reproducible solid-state FLIM calibration optimized for mid-range and portable imaging systems.
Applications
- FLIM-based clinical and preclinical system validation
- Training environments and educational FLIM labs
- Imaging platforms with limited spatial resolution
- Software benchmarking and algorithm development
- Longitudinal studies in drug screening and cell metabolism
- Low-cost instrument manufacturing and quality assurance
Close matching fluorophores
BPXS1
Alexa 350, Pacific Blue, Hoechst 33342, DAPI, NADH, Pyroxidane*, Collagen*, Elastin*
BPXS3
Porphyrin, Alexa 568, Propidium, mRFP
BPXS4
Flavin, Lipopigments, Calcein, Acridin Orange DNA, ATTO 488, BODIPY – FL, EGFP, FITC, Alexa 448, Eosin, SYTO 14, FAD, SYTO 11, SYTO 13, SYTO 16, TOTO – 1
BPXS5
Rhodamine B, Alexa 555, Tetramethylrhodamine, Cy3
*spectra mimics above 400 nm
Whether you’re calibrating an entry-level FLIM system, running educational training, or managing QC on a tight budget, our Low Resolution Fluorescence Lifetime Imaging Test Targets provide unmatched stability, traceability, and ease-of-use in a compact and cost-effective format. Built for reliability—priced for accessibility.
Looking for high resolution fluorescence lifetime test targets? Click here!
For an overview of our fluorescence test targets, learn more here!
